The following diagnostic tests are performed on request at the D7 Anatomical Pathology laboratory, NHLS
ALCIAN BLUE for acid mucins
ALCIAN BLUE-PAS for acid- and neutral mucins
ALIZARIN RED S for calcium
AYOUB-SHLAR for keratin
BEST’S CARMINE for glycogen
BILE SIRIUS RED for bile and collagen
BISMARCK BROWN for mast cells
BROWN AND BRENN for Gram-positive and –negative bacilli
CONGO RED for Potassium permanganate
FITE’S MODIFIED ZIEHL-NEELSEN for mycobacteria, especially leprosy
GIEMSA – KIEL METHOD for inclusion bodies, toxoplasma and CMV
GOMORI’S METHENAMINE-SILVER for basement membrane
GOMORI’S ONE-STEP TRICHROME for connective tissue and muscle
GORDON AND SWEET’S for reticulin fibres
GRIMELIUS for argyrophil granules
GROCOTT’S GOMORI’S METHENAMINE SILVER for fungi
HAEMATOXYLIN AND EOSIN for nuclei and general tissue architecture
HALE’S DIALYZED (COLLOIDAL) IRON for acid mucins
HIGH IRON DIAMINE for sulphated- and sialo- acid mucins
HIGHMAN’S CONGO RED for amyloid
HYALURONIDASE for the removal of hyaluronic acid
LENDRUM’S PHLOXINE TARTRAZINE for viral inclusion bodies
LONG ZIEHL-NEELSEN for lipofuscin
LUXOL FAST BLUE – MODIFIED for myelin
MALLORY’S PHOSPHOTUNGSTIC ACID HAEM for neuroglial fibres
MARTIUS SCARLET BLUE for fibrin
MASSON FONTANA for argentaffin granules
MASSON FONTANA for melanin
MASSON’S TRICHROME for collagen and muscle
MELANIN BLEACH for removal of melanin
MILLER’S VICTORIA BLUE for elastic fibres
MODIFIED GIEMSA for Helicobacter pylori
OIL RED O for lipids
PALMGREN for nerve fibres
PERIODIC ACID SCHIFF’S for carbohydrates
PERIODIC ACID SCHIFF’S DIASTASE for removal of glycogen
PAS-OG for anterior pituitary cells
SOLOCHROME CYANINE for myelin
SOUTHGATE’S MUCICARMINE for mucin
SULPHATED ALCIAN BLUE (SAB) for amyloid
THIO-T for amyloid
UNNA PAP/ DALES METHYL GREEN-LIGHT-PYRONIN for RNA (plasma cells)
VAN GIESON for collagen
VERHOEFF’S ELASTIC VAN GIESON for elastic fibres
VON KOSSA for calcium deposits
WARTHIN STARRY for spirochaetes; cat scratch disease
ZIEHL-NEELSEN for acid fast bacilli