Functional annotation of human long noncoding RNAs via molecular phenotyping
The FANTOM (Functional ANnoTation Of the Mammalian genome) is a worldwide collaborative project aiming at identifying all functional elements in mammalian genomes. The goal of the sixth edition of the FANTOM project (FANTOM6) is to systematically elucidate the function of long non-coding RNAs (lncRNAs) in the human genome. Find out more - https://fantom.gsc.riken.jp/6/
Long noncoding RNAs (lncRNAs) constitute the majority of transcripts in the mammalian genomes, and yet, their functions remain largely unknown.
Reto Guler and Frank Brombacher and co-authors as part of the FANTOM6 project “systematically knocked down the expression of 285 lncRNAs in human dermal fibroblasts and quantified cellular growth, morphological changes, and transcriptomic responses using Capped Analysis of Gene Expression (CAGE)”.
“Antisense oligonucleotides targeting the same lncRNAs exhibited global concordance, and the molecular phenotype, measured by CAGE, recapitulated the observed cellular phenotypes while providing additional insights on the affected genes and pathways”.
In the paper, the consortium disseminate the largest-to-date lncRNA knockdown data set with molecular phenotyping (over 1000 CAGE deep-sequencing libraries) for further exploration and highlight functional roles for ZNF213-AS1 and lnc-KHDC3L-2.
Read the paper - Functional annotation of human long noncoding RNAs via molecular phenotyping
Article by Bonamy Holtak