Research Day 2nd Prize-Poster Presentation

SUB-MIC LEVELS OF NITRIC OXIDE AND HYDROGEN PEROXIDE IN MYCOBACTERIUM SMEGMATIS: A PROTEOMIC TIME COURSE INVESTIGATION

Naadir Ganief, Jessica Sjoureman, Nelson Da Cruz Soares, and Jonathan M. Blackburn

Division of Chemical and Systems Biology, Department of Integrative Biomedical Sciences, FHS, University of Cape Town.
The macrophage mediated oxidative burst is known to be an important mechanism for the host defence against mycobacterial infection. Two products of the oxidative burst are hydrogen peroxide and nitric-oxide. Here we have investigated the effects of sub-MIC hydrogen peroxide and nitric-oxide over time via liquid chromatography couple tandem mass spectrometry based proteomics. We have, with high confidence identified and quantified 3333 proteins and shown 176 and 259 proteins are significantly dysregulated for hydrogen peroxide and nitric oxide treatment respectively. Bioinformatics analyses revealed the treatments shared some responses, notably both treatments induced the dosR response, altered signalling via two component systems as well as dysregulated lipid metabolism. The treatments also elicited unique responses in amino acid metabolism their metabolism with hydrogen peroxide treatment resulting in dysregulation of the leucine, isoleucine and valine metabolism, whereas nitric-oxide treatment resulted in dysregulation of glutamate metabolism.