IDM Microscopy

We offer training and user support for a fleet of microscopes across BSL2 and BSL3 settings. In partnership with Africa Microscopy Initiative (AMI), we also facilitate training and access to the microscopes in the AMI Imaging Centre for local users. Find out more about the AMI Imaging Centre microscopes here.

IDM-µ Microscopes

Name Description Status
Zeiss AxioScan 7 Slide scanner. This is an upright transmitted light and wide-field fluorescence microscope. The automated slide loader means the microscope can process up to 100 slides in one run. Available for booking
Objectives Installed:
5x/0.25 NA Fluar
10x/0.45 NA Plan Apo
20x/0.8 NA Plan Apo
40x/0.95 NA Plan Apo
Filters Available:
Filter set 91 HE
Filter set 112 HE
Light Sources:
Transmitted light: WL-LED
Fluorescence: Colibri 7 (FR-R[G/Y]BV-UV)
Detectors:
Axiocam 705 colour
Axiocam 712 mono
Zeiss Elyra S1 (BSL3) Structured illumination super-resolution fluorescence microscope, housed in BSL3 laboratory. Available for booking
Objectives Installed:
10x/0.3 NA DICI EC Plan Neofluor
40x/0.6 NA Ph2 LD Plan N
63x/1.4 NA (Oil) DICII Plan Apo
63x/1.2 NA DICII C Apo
100x/1.4 NA (Oil) DICII Plan Apo
Filters Available:
Light Sources:
WFL HXP 120 V UV lamp
Lasers: 405 nm, 488 nm, 561 nm, and 642 nm
Molecular Devices IXM-C (BSL3) A high-content inverted microscope housed in BSL3. This system can image live samples with transmitted light, wide-field fluorescence, and spinning disc confocal microscopy. Available for booking
Objectives Installed:
10x/0.45 NA Plan Apo Lambda
10x/0.3 NA Ph1 DL (dark low) Plan Fluo
40x/0.95 NA DIC N2 Plan Apo Lambda
60x/0.95 NA DIC N2 Plan Apo Lambda
20x/0.45 NA S Plan Fluor ELWD
4x/0.2 NA Plan Apo
Filters Available:
Light Sources:
Lasers: 405 nm, 488 nm, 561 nm, and 642 nm
Detectors:
Andor Zyla sCMOS
DeltaVision Wide-field inverted microscope, equipped for live-cell imaging and deconvolution. Not Available for booking
Objectives Installed:
Filters Available:
Light Sources:
Detectors:
M2 Aurora Simple lightsheet microscope, with non-scanning scanning illumination, water-dipping objectives, optimized for imaging small spheroids and organoids. Not Available for booking
Objectives Installed:
Illumination: 10x/0.3 NA UMPlanFL (water)
Detection: 20x/0.5 NA UMPlan FL N (water)
Light Sources:
Lasers: 405 nm, 561 nm
Optical BioSystems StellarVision A high-content microscope offering increased resolution at 20X by Synthetic Aperture Optics. This microscope is designed for use in spatial 'omics imaging and other FISH applications. Not Available for booking
Objectives Installed:
20x/0.45 NA S Plan Fluor
Light Sources:
Lasers: 405 nm, 488 nm, 561 nm, and 647 nm

IDM-µ Services:

  • Comprehensive imaging project consultations

  • Guidance on experimental design and planning

  • Microscope user training and support

  • Sample preparation training

  • Image analysis guidance

We are open to collaborations - discuss your project with us.

More extensive project input and support, from assay design through to image analysis, can be negotiated on a collaborative basis.